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The molecular and ultrastructural profiles of the symbionts found in the midgut and ileum of Cephalotes atratus, Cephalotes clypeatus, and Cephalotes pusillus were determined using the V3 region of the bacterial 16S rDNA gene and transmission electron microscopy (T.E.M.). Two samples of C. atratus, three of C. clypeatus, and six of C. pusillus were analyzed. The coefficients of similarity ranged from...
An automatic coarse-alignment method for a tilt series of rod-shaped specimen collected with a full angular range (from α=−90° to +90°, α is the tilt angle of the specimen) is presented; this method is based on a cross-correlation method and uses the outline of the specimen shape. Both the rotational angle of the tilt axis and translational value of each image can be detected in the images without...
In this work, a method to determine the atomic ratio of Mo and C within complex metal carbides using EELS in the TEM has been developed. The method is based on the determination of k-factors for given experimental conditions from the EEL spectra of Mo 2 C and MoO 3 standards, which had been independently checked by XRD and EPMA. Factors affecting the k Mo/C value of the Mo...
Image quality of MeV transmission electrons is an important factor for both observation and electron tomography of microns-thick specimens with the high voltage electron microscope (HVEM) and the ultra-HVEM. In this work, we have investigated image quality of a tilted thick specimen by experiment and analysis. In a 3MV ultra-HVEM, we obtained transmission electron images in amplitude contrast of 100nm...
In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex developmental...
Bancroftian filariasis constitutes the principal mosquito-borne nematode infection of humans and the surface of adult of Wuchereria bancrofti seems to be especially important in the intricate interplay between host and parasite. The study of the parasite's surface structure might help to understand the localization and function of various organelles. W. bancrofti adult worms were recovered from untreated...
The oxygen distribution in Ni 2 Si and NiSi films formed during a two-step silicidation process was analyzed by time-of-flight secondary ion mass spectrometry (TOF-SIMS). TOF-SIMS mass spectra revealed that both silicon and nickel reacted with oxygen at the Ni 2 Si surface. In addition, silicon nitride was formed at the surface by the reaction of silicon with nitrogen in the TiN capping...
Effects of electron irradiation damage in γ-LiAlO 2 were analysed using energy loss near edge fine structure (ELNES) of the O K-edge. Simulations of the O K-fine structure by means of a density functional theory (DFT) code show that under the electron beam γ-LiAlO 2 undergoes the following transformation: LiAlO 2 → LiAl 5 O 8 → Al 2 O 3 . O K-edge...
Thermal martensite characteristics in Fe–29%Ni–2%Mn–2%Co alloy were investigated with scanning electron microscopy (SEM) and Mössbauer spectroscopy characterization techniques. SEM observations obviously revealed the lath martensite morphology in the prior austenite phase of examined alloy. As well, the martensitic transformation kinetics was found to be as athermal type. On the other hand, Mössbauer...
We report on the surface potential characteristics in the equilibrium state of the grounded insulating thin films of several 100nm thickness negatively charged by a low-energy (<5keV) focused electron beam, which have been simulated with a newly developed two-dimensional self-consistent model incorporating electron scattering, charge transport and charge trapping. The obtained space charge is positive...
The main criterion to classify a microorganism as belonging to the genus Beggiatoa is its morphology. All multicellular, colorless, gliding bacterial filaments containing sulfur globules described so far belong to this genus. At the ultrastructural level, they show also a very complex cell envelope structure. Here we describe uncultured vacuolated and non-vacuolated bacteria from two different environments...
The extracellular matrix (ECM) consists of various types of protein and carbohydrate polymers with red-ox and acid–base properties that have a crucial impact on tissue homeostasis. In the present study, a combination of both frequently applied and also specialized histochemical staining methods were used to reveal the chemical properties of the ECM of the snail central nervous system (CNS) which has...
Morphological studies in male reproductive system of insect, and accessory glands functions, can contribute to the knowledge of reproductive aspects in Triatominae. The present study aims to describe the structure of male reproductive system under scanning electron microscopy, and the ultrastructure of accessory glands during the process of maturation, in T. brasiliensis and T. melanica of different...
In recent years, experimental and clinical evidence has been provided regarding endothelial dysfunction and its contribution to the inflammatory process leading to atherosclerotic plaque formation. All the techniques employed direct to the study of endothelial dysfunction, however usually require isolation of endothelial cells and therefore tissue manipulation and destruction, with subsequent loss...
The aim of this study was to examine the effect of fluoride application on 37% phosphoric acid-etching by atomic force microscopy (AFM) in primary tooth samples based on a clinical protocol used in a pediatric dental hospital. Enamel samples were prepared from 36 exfoliated and non-carious primary teeth. Primary tooth samples were randomly assigned to one of the four groups based on the timing of...
An outdoor soil burial test was carried out to evaluate the degradation of commercially available LDPE carrier bags in natural soil for up to 2 years. Biodegradability of low density polyethylene films in soil was monitored using both optical and scanning electron microscopy (SEM). After 7–9 months of soil exposure, microbial colonization was evident on the film surface. Exposed LDPE samples exhibit...
An artificial convoluted tubular system with precise predefined parameters was created. It was stereologically analyzed in order to preview the potential probability to introduce errors when applied to biological systems like testicular tubes or glomerule like structures. The length of the convoluted tubules more frequently analyzed was estimated by five different methods. The analytical methods were...
This study reports a determination of the polarity of WC facets in WC–Co alloys sintered at the liquid state. In these alloys, WC grains are delimited by basal facets and two sets of {1 0 1¯ 0} prismatic facets, one set of facets being much more developed than the other. A variation of the shape is observed as a function of the composition of the alloy. High resolution transmission electron microscopy...
In this research the spatial distribution characterization of niobium phosphate into bleached cellulose was carried out combining processing and images analysis obtained by SEM and statistical methodologies. The objective is to investigate the deposit composition and phosphate morphology by using complementary analytical techniques. Based on the proposed methodology, parameters of niobium phosphate...
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